Impact of Bosentan in Ameliorating Methotrexate-Induced Tongue Toxicity in Rats

Section: Research Paper
Issue
Vol. 22 No. 1 (2025): Volume 22 Issue 1
Published
Mar 1, 2025
Pages
8-13

Abstract

Background and objective: Although methotrexate has been utilized over many years, adverse effects are frequent throughout therapy, especially among cancer patients. The study examined the efficacy of bosentan as a protective therapy against tongue toxicity induced by methotrexate. Methods: This study employed twenty-four 300400 g Wister-albino rats. The animals were categorized into three groups: methotrexate (80 mg/kg), methotrexate (80 mg/kg) plus bosentan (60 mg/kg), and control healthy (distilled water). The tongue mucosa was subjected to biochemical and immunohistochemical analysis. The animals' tongue mucosa was dissected immediately following they were euthanized on day 16. Results: In contrast to the control group, the Methotrexate group had significantly higher levels of TNF-, IL-1 and MDA (p < 0.05). However, the Methotrexate+Bosentan group displayed considerably lower levels of TNF-, IL-1 and MDA in contrast to the Methotrexate group, (p < 0.05). Conclusion: These findings demonstrate that bosentan protect considerably against methotrexate-induced tongue toxicity in rats.

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Author

Rana Khairi Attarbashee

Department of Dental Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq

ORCID

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Khairi Attarbashee, R. (2025). Impact of Bosentan in Ameliorating Methotrexate-Induced Tongue Toxicity in Rats. Iraqi Journal of Pharmacy, 22(1), 8–13. Retrieved from https://rjps.uomosul.edu.iq/index.php/iphr/article/view/21560