Measurement of serum zinc level in patients with warts, cardiovascular diseases, and leukemia

Objectives: To evaluate serum zinc (Zn) level in patients with warts, cardiovascular problems, and leukemia.Patients and methods: One hundred eighty six patients with warts, cardiovascular problems, and leukemia; plus 30 apparently healthy volunteers were included in this study. The patients were classified according to their clinical conditions to three groups. Seventy three patients with skin warts, 78 patients with cardiovascular diseases (CVD), and 35 patients with leukemia. Blood samples were taken from patients and controls. The blood was placed in zinc free plastic tube left for 45 minutes, then separated by centrifuge to obtain the serum. To one ml of the serum, 9 ml of 25% trichloroaceticacid were added and shaken for 10 seconds. Then the samples were put in a water bath at 37 C for 10 minutes, centrifuged and the supernatant was transferred into a clean plastic tube, each sample was aspirated into atomic absorption spectrophotometer and the element was measured.