Quantitative and Qualitative Determination of Rutin and Quercetine in Callus Resulting from Parts of Citrullus colocynthis L. Using the High Performance Liquid Chromatography HPLC

The present study arrived at initiated callus from Citrullus colocynthis L. namely from these parts (leaves, stems, cotyledons and hypocotyls)on the solid MS medium supported with different concentrations of auxins and cytokinins. The originate callus average estimated at 100% in all plant parts under study in the MS medium provided with 1.0 mg/L BA+0.5 mg/L NAA. Therefore, this was selected as the best medium to product callus from the plant. The quantitative and qualitative measuring of alcohol extract of callus resulting from Citrullus colocynthisL.utilizing the HPLC technique showed that it contains the highest concentration of the Rutin compound. This was amounted to 40.613% before hydrolysis similarly the highest concentration of the quercetine compound was amounted to 39.182% in this extract after the hydrolysis.
Key wordes: Citrullus colocynthis, plant tissue culture, high performance liquid chromatography, phenolic compound.