Utilization of palm syrup(Dibs) and honey as carbohydrate on initiation and growth of callus Coriandrum sativum L.
Abstract
() (Coriandrum sativum L.). (MS) (BA,NAA) 1.0 / . ( ) . 2 /100 3.630 130 (4,6) /100 30 1/100 MS (2.216) (4,6) / 100 (0.700 0.500) . . (2.460) / 6 /100 . The present study included the effect of date palm syrup (Dibs) and honey as the sole carbohydrates source on the initiation and growth of callus Coriandrum sativum L. leaves using Murashige and Skoog (MS) medium that contains 1.0mg /L of (BA,NAA) .the results cleared that date palm syrup and honey have capable of callus initiation and growth as carbon and energy source instead of sucrose usually used in most plant tissue culture .The result indicated that addition (Dibs) of 2ml/100ml to the nutrient medium induced the initiation of the callus with higher than that in the presence of sucrose, the fresh weight reaches to 3.630g after 130 day of culturing .whereas adding of high concentrations of dibs (4,6)ml/100ml could not stimulate of the callus. Initiation and results indicated also that adding different concentrations of honey would induce ,but to a very little proportion that grown as compared with the addition of (30)g of sucrose (adding 1ml/100ml of honey induce callus growth similarity to the callus growing in (MS) medium that contains sucrose (the fresh weight reaches to 2.216g).whereas adding high concentrations of honey does show a decrease in the induction of the callus (4,6)ml/100ml , the fresh weight reaches to (0.573-0.700 g).The study also showed that the carbohydrate content of the callus grown in nutrient medium which contains different concentrations of honey may surpass that content of the callus grown in both sucrose and dibis .such a content shows a highest proportion of carbohydrate (2.460)mg/g for the callus grown in honey of concentrations (6ml/100ml) of nutrition medium.