EFFECT OF SPERM CAPACITATION MEDIA ON IN VITRO FERTILIZATION OF ARABI EWES OOCYTES

The study was conducted at Assisted Reproductive Technology Center (A R T) College of Medicine, University of Basra , from January , 2009 to January ,2010 . The aim was to study the effect of capacitation media on in vitro fertilization of Arabi ewes oocytes . A total of 2279 oocytes were collected from ovaries obtained from Basra Slaughter House .Oocytes were matured in TCM199 (tissue culture medium 199) supplemented with10%Fetal bovin serum (FBS) to study the effect of capacitation media on their fertilizing ability. freshly ejaculated and frozen thawed spermatozoa were capacitated in 1 of 2 media .A Bracket culture medium or Earles balanced salt medium . Results revealed that Bracket medium with Hyalouronidase enzyme and Gentamycin instead of Penicillin antibiotics showed significant increase (P 0.05) in number of in vitro fertilized and divided ewes oocytes as compared with the Earles in both fresh and frozen thawed spermatozoa .