ANTIBACTERIAL ACTIVITY OF Achillea fragrantissma L. AND FRUITS OF Citrus aurantifolia L. EXTRACTS AGAINST SEVERAL GRAM POSITIVE AND NEGATIVE BACTERIA

Biological activities of Citrus aurantifolial and Achillea Fragrantissmal extracts were tested against two species of Gram positive bacteria , Staph. lococcus aureus , Bacillus subtilis and four species of Gram negative bacteria , Pseudomonas aeruginosa , Proteus vulgaris , Kiebsilla pneumonia , Escherichia coli . Fruit of Citrus aurantifoliaL. and flowers of Achillea FragrantissmaL. were extracted using Dist water and Soxhlet apparatus in presence of Ethanol , Chloroform , and Aceton . Water extract of Achillea FragrantissmaL. showed no activity against all bacterial species tested , but the water extract of Citrus aurantifoliaL. showed a good antibacterial activities using disc diffusion method and comparing with the standard antibiotic (Gentamicin and Tetracycline ) . Result also indicated that ethanol extract showed high antibacterial activities in all types species tested . Chloroform extract of Citrus aurantifoliaL. and Acetone extract of Achillea FragrantissmaL.on the other hand, showed no activity on all species of bacteria tested , The study also detected the Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of the two plant extracts , tested bacterial showed high sensitivity against acetone extract of Citrus aurantifoliaL. , Staph. aureus , B.subtilis , Pr.vulgris and E.coli was more sensitive than other tested bacteria , the calculated (MIC) reached 0.125 mg/ml while the ( MBC) reached 0.5 mg/ml .