CHROMATOGRAPHIC SEPARATION AND IDENTIFICATION OF SOME PHENOLIC AND CARBOXYLIC ACIDS OF Populus nigra L. BARK GROWING IN NINAVAH PLANTATION

Separation of some phenolic and carboxylic acidsfrom the bark of Populus nigra L.trees was carried out. Sequence of solvents system extraction by two different polar solvents (Benzene and Ethanol 95%) was done by using the Soxhlet in order to prepare crude extract. HPLC was used for separation, identification and also determination of some phenolic and carboxylic acids and its percentages .The results showed a remarkable variation in the kind, number, percentage and the peak area (represented the concentration) of each separated compound. Oxalic acid, Acetic acid, Acetylsalicylic acid and Cinnamic acid were identified.