INFLUENCE OF CULTURE MEDIA AND DENSITY OF SOYBEAN CELL SUSPENSION CULTURE BY MULTIPLE DROP ARRAY ON FORMING CALLUS PRIMORDIA

The present study established cell suspensions from the friable callus derived from the stem explant of soybean (Glycine max L.) seedlings. These suspensions of certain densities were cultured by embedding in agar using MS & B5 media supplemented with favorable level of Naphthalene acetic acid (NAA) and Benzyl adenine (BA) using Multiple Drop Array (MDA) technique. Culture density reached up to 7.3105 cell/ml with viability between 47-81% and 4.1105 cell/ml in B5 medium. Colonies were developed to form callus primordia and continued their subsequent growth so they increased in size to form small piece of callus in agar drops reached up to 85% in MS medium.