PRODUCTION OF CARDAMOM PLANTS ( Elettaria cardamomum Maton.) IN ONE – STEP REGENERATION OF EMBRYOS STIMULATED IN VITRO

stimulated embryos were excised from Elettaria cardamomum Maton. Seeds. The best of these were obtained from micropropagation MS + 2,4-D 5.0 mg/L for 20 days, and grown on the initiation medium MS+ BA 4.0 mg/L+NAA 1.0 mg/L.They were differentiated to form plants in onestep regeneration with a number of 54 plants in an average of 18 plants/callus piece after 210 days of culture and in a response percentage of 75%.Cardamom seed embryos, stimulated on the medium MS+2,4-D 25.0 mg/L for 20 days and subsequently cultured on initiation medium MS+BA 2.5 mg/L + NAA 1.0 mg/L.They showed an ability to produce callus which were differentiated to plants in onestep regeneration earlier in comparision with a number of 45 plants, at an average of 15 plants/callus piece after 210 days of culture and in a response percentage of 60%.This study succeeded in obtaining callus from immature embryos, removed from cardamom seeds, stimulated by the medium MS+2,4-D 20.0 mg/L and then grown on initiation medium MS+BA 1.5 mg/L +2,4-D 1.0 mg/L after 52 days post- culture in a percentage of 50%.The best of average fresh weight of callus was 2.76 gm after 120days of culture. The regenerated plants from tissue culture were successfully acclimatized after being hardened.