Expression of miR-571 and miR-20a in Breast Cancer Patients as Diagnostic Biomarkers

Micro RNAs (miRNAs) are small nucleic acids and they are non-coding type of RNA, which are composed of 19-25 nucleotides and work as significant post-transcriptional gene controls of multiple biological roles. Commonly, miRNAs negatively control gene expression by adhering to their particular messenger RNAs in which they generally bind to the 3'-UTR (UN translated region) of their target mRNAs and repress protein production by destabilizing the mRNA and translational silencing. (mRNAs), for both mRNA degeneration or translational restraint, depending on the level of their complementation with target its mRNA chains. Unusual expression of those miRNAs was found to be associated etiologically with several diseases such as breast carcinoma. Diverse cellular pathways involved in breast cancer is developing such as cell proliferation, apoptosis,metastasis, chemoresistance, and cancer recurrence which are controlled by the oncogenic miRNA (oncomiR) or suppressor miRNA for tumor (tsmiR). In the present study, the representation levels of miR-571 and miR-20a, the most two studied miRNAs in breast cancer, were estimated in 40 breast cancer patients at Hiwa Hospital, Sulaimani city. Real-Time-Reverse Transcription-PCR (RT-PCR) was applied to evaluate the expression of miR-571 and miR-20a. The clinical data including breast cancer with different grades, a cancer patient with chemotherapy state ( pre-chemotherapy), and ( post-chemotherapy) conditions. Results exhibited that both miR-571 and miR-20a expression is correlated with disease stage among patients taking chemotherapy. According to the results, chemotherapy increased the expression of miR-571, but reduced the expression of miR-20a in patients with breast cancer. We also found that chemotherapy had more effect on miR-571 expression in comparison with miR-20a. Furthermore, the effect of chemotherapy was higher in the early stages of breast cancer in comparison to other disease stages. In the coclusionusing both miR-571 and miR-20a as important biomarkers for detecting the the beneficial effects of chemotherapy on breast cancer.