Gene Expression Profile Of Lncrna Meg3 In Prostate Cancer And Normal Cell Lines
Abstract
Background and Aim: Cancer is one of the major of human problems and prostate cancer is a popular healthy problem in cancers. In spite of the developing treatment methods, prostate cancer cannot be treated effectively.Therefore, the identification of novel genes that will be role in the treatment and diagnosis of prostate cancer is of greatest interestmeg3 (meg3 family tumer suprosser gene are key regulators of programed death cell . Objective Although studies have saw that this meg 3 has processes, its usefulness in the treatment and diagnosis of prostate cancer has not been fully elucidated.At the same time, previous studies have shown that the meg 3gene is associated with important protein apoptosis triggering proteins such as HAGLR and TSIX. In this study, by using gene expression level, we aimed to investigate the relationship between the levels of meg3,and haglrgene which the prognosis and apoptosis processes of prostate cell line. Materials and Methods: Collected of the sample from normal and cancer tissue type in the cell line, RNA isolated from the cell, RNA quantitation, cDNA synthesised, cDNA quantitation ,and Real -time PCR was performed to see the level between two types of genes. Results: meg3 tumer supresur level was significantly lessened in regulator molecule tissues of a cell line with prostate cancer . In contrast to HAGLRprotein, no significant difference was detected in TSIX expression level. However, Our study also showed that low meg3 expression level is associated with important clinic characteristics of the patient such as tumor grade, stage and breast cancer type. Conclusion: Findings of our study show that meg3 has roles in prostate cancer formation and might be a novel biomarker for the diagnosis and treatment of cancer.
References
- .
- Meg3 tumer suppruser gene are classified in three subfamilies. The meg3 and HAGLR, are of high interest for cancer treatment, with major key questions yet to be elucidated
- . FOXAl regulatory plexus harboring somatic singlenucleotide variants in primary prostate tumors
- . Genome sequencing and gene expression analyses of prostate tumours
- . In this study, determination of expression profile of MEG3 gene in various cancer and normal cell lines was aimed in order to lead more information about its function in cancer.
- In many types of cancer, meg3 inhibiting the expression of sense gene , leading to disruption of the linc RNA mechanism
- . in this study, we aimed to reveal the roles of meg3, HAGLR ,TSIX , which play roles in prostate cancer progress. Healthy tissues of patients were used as controls. Particularly, meg3 gene can expressed levels were significantly increase in tumor tissues of prostate cancer patients (p = 0.0078). In addition, there was significant difference in the expression level of HAGLR gene between normal and tumor tissues patients (P= 0.0001)in prostate cancer figer (2). In addition, High-risk disease accounts for approximately 15% of prostate cancer diagnoses
- . MEG3 is suggested to function as a tumor suppressor lncRNA
- . Meg3 one of the key molecules that lead to multidrug resistance to the cell,. (MEG3) (lncRNA) located on chromosome 14q32.3.
- . Precision medicine for advanced prostate cancer may identify new treatment strategies and change clinical
- practice
- . prostate cancer that facilitates tumor formation, disease progression and therapeutic resistance
- . The excess level of meg3 inhibits cell death whereas low levels of meg3 increase the susceptibility of the cell to various toxins.
- . The therapeutic landscape of prostate cancer has been transformed over the last decade by new therapeutics,
- .. Recent studies show that several cancer risk loci are transcribed into lncRNAwhich plays key roles in tumorigenesis
- .HAGLR is aLong non-coding RNAs , have limited or no protein- coding , regulation network of cancer and play an important role in tumourigenesis and progression
- .In this study that regulate cancer cell MEG3 and HAGLR may have important roles for several tumorigenic processes. To explore their roles, ATCC normal and cancer (PC,) cell lines were subjected to examine through RNA isolation, cDNA conversion, quantitative RT-PCRfor expression analyses. (MEG3 and HAGLR) genes have differential expression pattern in both normal and cancer cell lines. The obtained results indicate their importance for biological processes in cancer. Tsix is instead required to silence Xist on the active-X
- .The dysregulation and alteration of several lncRNAs has been reported in various cancer types. Based on the expression lncRNAs,expression profiling of human tumors as identified marks associated with diagnosis, staging, progression, prognosis, and response to treatment
- .where it may not cause serious harm. However, while some types of prostate cancer grow slowly and may need minimal or even no treatment, other types are aggressive and can spread quickly.
- Aims: A substantial fraction of cancer's LncRNAs exert diverse biological functions by regulating gene expressions and functions
- are new players in gene regulation but their mechanisms of action are mainly undocumented
- Genetic variation at 8q24 is a major contributor to prostate cancer (PCa)
- The HOXD-AS1 (also known as HAGLR gene is located between the HOXD1 and HOXD3 genes in the HOXD cluster
- TSIX siRNA reduced the mRNA expression of type I collagen