Distribution of Her2 Single Nucleotide Polymorphism in Different Geographical location in Sulaimani Province,

ABSTRACT Her2 single nucleotide polymorphism is known to be associated with the risk of breast cancer in women. The distribution of mutations in the Her2 gene differs from one geographic location to another. This study aimed to find two risky genotypes of Her2, Ile/Val Val/Val, in different geographical regions of Sulaimani province. For this purpose, 272 blood samples were collected from healthy women and women who have breast cancer for genetic analysis. The detecting alleles were performed by PCR amplification of the her2 gene and then digestion of the DNA by enzyme BsmAI to find different fragments of DNA representing different genotypes of Ile/Val, Val/Val. Among 272 cases, it was found that the highest rate of single nucleotide polymorphism was in Sulaimani City, 38.9% and the lowest rate of mutation of Her2gene was found in Raparin, 1.8%. The distribution of Ile/Val and Val/Val in other regions was as follows: Garmyan, 6.6% and Halabja, 2.2%. At the same time, the correlation between chronic diseases with breast cancer and with her2 single nucleotide polymorphism was studied. It was found that there were no significant relationships between chronic diseases and Her2 mutation. It concluded that risky genotypes, Ile/Val Val/Val, exist in Sulaimani province, and the highest rate was detected in Sulaimani city.