The Influence of Natural Products as Denture Cleansers on Candida albicans Colonization to Cobalt–Chromium Alloy Denture Base Material.

Aims: The study aims to evaluate the influence of various denture cleansers on colonization of Candi-da albicans to CobaltChromium alloy denture base and the subsequent roughness assosiated with these cleansers. Materials and methods: Thirtysix samples of CoCr denture base and five cleansers, four prepared(alum, salt, soda+vinegar, soda+thymol), one commercial ( protefix ) and distilled wa-ter(D.W) as a control were used. Samples were immersed in denture cleansers for one month in which each cleanser had 6 samples excluding 6 samples in D.W as a control. Half of samples for each cleans-er were immersed hr per day and the other half immersed 8hrs per day through one month, before microbiological examination, samples were tested for surface roughness using profilometer. Candida albicans cell suspension was incubated with the test samples for 1hr at 37C after which the test sam-ples were immersed in their cleansers for 1hr. Visualization, inspection and enumeration of adherent C.albicans cells and detection of the antiadherent effect of the cleansers was achieved by using light microscopy. Results: The results demonstrated insignificant difference in surface roughness of CoCr alloy denture base in the cleansers at 1/2 hr and a significant difference at 8 hrs immersion. There was a significant difference in C.albicans colonization to CoCr denture base in which all the cleansers showed less adhesion than control. The results also revealed that (soda+Thymol oil) cleanser ex-pressed the least values of colonization and roughness among other cleansers. Conclusions: The cleansers were effective as antiadherent yeast cells to CoCr denture base and showed roughness de-grees less than control