Effect of Different Nanoparticles Incorporation in Acrylic Based Soft Liner on Candida albicans Adhesion
Abstract
Aims: To evaluate the effect of the addition of MgO, ZrO2, ZnO nanoparticles in different concentration to acrylic based soft liner on candida Albicans adhesion. Materials and methods: acrylic-based soft-liner samples were prepared (10 X10 X 2mm dimension) by addition MgO, ZrO2, ZnO nanoparticles in 0.25, 0.5, 1, 2, 4 % by weight concentration to the monomer resin, the samples were immersed in Candida Albicans suspension for 1 hour then washed and dried, the microorganism were fixed and stained by crystal violet and examined by light microscope (at 400 x) to enumerate the stained candida Albicans in two occasions for thirty fields of view. Result: soft liner with 1,2,4 % by weight demonstrated that a decreased the surface adhesion of candida Albicans when compared with control group while specimens with lower concentration (0.25, 0.5% by weight) show no significant difference from control. Conclusion: addition of nanoparticles in 1,2,4 % concentration of weight to acrylic based soft liner impaired the adhesion properties of Candida Albicans, and reduce fungal induced denture stomatitis.
