Effect of Interaction Between Growth Regulators and Sulfanilamide on Stem Parts Callus Initiation and Suspension Culture of Almond Plants

Callus growth was affected by the types and concentration of growth regulators used. The best medium sustained callus growth was MS medium supplemented with 10-4 molar NAA and 10-5 molar BA. The callus fresh weight in such medium reached about 3.73 gm after 60 days of subculture as compared with other medium used. The growth of the cultures was affected by the type and concentration of growth regulator used as well as the sulfainlamide added. The best medium sustained suspension cultures growth was MS containing 10-8 molar NAA and 10-8 molar BA with cell density reaching about (53.5x105 Cell/Cm3) after 7 days of incubation. Callus initiation was about (%50.2) from the cells embedded in agar as compared with %80.31 by the addition 100 M sulfanilamide to MS medium containing 10-4 molar BA.