Tissue Culture Study of Ruta graveolens L. and Comparison of Dictamine Alkaloid in its Extracts

The study showed a variety in the production speed of callus of Ruta graveolens L. from different explants (stems, leaves and petioles), the better and fast in initiation and growth of callus were the stems then petioles and finally the leaves, the best media for initiation and production of callus with plentiful shoots was MS with (0.5,2.0)mg/L from BA and NAA respectively approach in results the best initiation media of stem callus (1.0,1.0) and the best initiation media of leaves callus (2.0,2.0) from BA and NAA respectively, whole plant was produced by rooting the shoots that formed from callus by using MS media with 0.5 mg/ L of NAA during 20 day, different extracts from (natural plant, callus and shoots regenerated from callus) varied in Dictamin alkaloid purity wich is noticed by measurement of IR , the best appearance was in callus and shoots extracts, bacterial sensitivity test proved by recording (13, 7mm) inhibition zone for Staphylococcus aureus induced by shoots extract callus extract respectively while the plant extract gave negative test