Effect of Passive Smoking on Some Physiological and Biochemical Parameters in Male Swiss Albino Mice ( Mus Musculus )

The study included the effect of passive smoking exposure for different periods on some physiological and biochemical parameters in the blood and organs of male mice, forty adult mus musculus male mice were used. All animals were randomly divided into five groups depending on exposure time to cigarettes smoke, the first group was exposed for two hours daily for two months, the second group was exposed for four hours daily for two months, the third group two hours daily for three months, the fourth group four hours daily for three months while the fifth group was considered as control group. Results showed a significant decrease (p < 0.05) in the total number of white blood cells and a significant increase (p < 0.05) in each of packed cell volume and heamoglobin concentration in groups exposed to cigarettes smoking as compared with control group.
The results also revealed that there is a significant increase in the activity of the enzymes: alanine amino transferase (ALT), aspartate amino transferase (AST), alkaline phosphatase (ALP), and gamma glutamyl transferase (GGT). The highest enzymatical activity occurred when increasing the exposure time to four hours for three months. A significant increase in bilirubin concentration was observed in groups that exposed for two hours, four hours daily for three months respectively. Results showed also significant decrease in protein concentration in the group which was treated for two hours daily for three months and four hours daily for three months as compared with the group that was treated with four hours daily for two months. Measurement of acetylcholineesterase activity in brain tissue showed a significant decrease occurred in the group exposed for two hours daily for three months when compared with the group that was treated with two hours daily for two months, and the group which was treated for four hours daily for three months as compared with the group which was treated for four hours daily for two months.
The conclusion from this study suggested that exposure to smoking for different periods causes alternations in some physiological and biochemical parameters in male albino mice.