Detection of the ability of isolated Enterobacter sakazakii from powdered milk for enterotoxins production

This study includes isolation & identification of bacteria from (35) different kinds of powdered infant formula milk involoved (Dialak 1-2, Celia 1, Hery 1-2, Biomil 1-2, Albadees 2, Nido 2, Kikose 2, Nictalia 1-2, Cerilak). The results show (10) different isolates of germs at apercentage of 28.57% distributed as 8.57% for Escherichia coli & 5.71% for each Enterobacter sakazakii and also for Klebsiella pneumoniae reguensee, while the isolates of Aeromonas veronii, Aeromonas hydrophilia, Shigella desentri was 2.85% for each one of them.
We detected toxins of Enterobacter sakazakii in eather cell free culture supernatant (CFCS) and also in lysate supernatant (LS) by used suckling mouse test which gives apositive result for Rapid & Delayed permeability test by appear non keratinize blue aerea on Rabbit skin for detection of Heat -Stable Enterotoxin and also used pow Oedema test for detection of Heat-Labile Enterotoxin for Enterobacter sakazakii which gives apositive result by appear biymp in anterior and posterior hand of mouse compired with the control.