Salicylic Acid Induced Tolerance of Cotyledons Callus of Linum usitatissimum L. to Culture Filterate of Fusarium oxysporum f.sp. lini

(MS ) Murashige & Skoog MS 1.0 / (BA) Benzyl Adenine Naphthalene Acetic Acid (NAA) . f. sp. lini Fusarium oxysporum 10 % 0.0 5.0 10.0 15.0 20 % . MS 1.0 BA / + 0.5 NAA / . MS . . 10 102 103 104 4 8 12 24 28 10 % 15 . 104 24 10 % 10 4 10 % . callus was stimulated from cotyledons explants of Linum usitatissimum seedlings on Murashige & Skoog (MS) media containing different concentrations of growth regulators. Medium containing 1.0 mg / L of Benzyl Adenine (BA) and Naphthalene Acetic Acid (NAA) was the best for callus induction. Following the testing of media containing the concentrations of 0.0 , 5.0 , 10.0 , 15.0 and 20.0 % culture filterate of an isolate of Fusarium oxysporum f. sp. lini . The medium containing 10.0 % culture filterate was selected as the best medium for regeneration of filterate tolerant callus. Regenerated stems formed roots on MS medium. Plants from tolerant callus exhibited resistance to toxic filterate callus grown on the medium containing 10% fungus filterate for 15 days following treatment with 10 , 102 , 103 and 104 mole of salicylic acid for 4 , 8 , 12 , 24 and 28 hours showed an increase in brown colour darkness with the increase of salicylic acid concentration and treatment period. The highest peroxidase activity was found in callus cells extracts treated with 104 mole salicylic acid for 24 h and cultured on the medium containing 10.0 % culture filterate, whereas catalase activity was decreased with the increase of salicylic acid concentration in the medium and the treatment period. The highest recorded enzyme activity was found when 10 mole / L salicylic acid was used for 4 hours with 10 % fungus filterate.