Isolation And Identification Of Some Enterobacterial Species From Children With Diarrhoea In Mosul And Studying Their Ability To Produce Protease Enzyme

50 2005 45 18(40%)E.coli 16 (35.5%) Klebsiella pneumoniae4 (8.8%) Proteus 3 (6.6%) Citrobacter (4.4%) Serratia Salmonella . Serratia marcescens Proteus mirabilis Salmonella Klebsiella pneumoniae .This study included the isolation and identification of some bacterial species causing diarrhea in children under five years old . Fifty stool specimens were collected from in and out patients of Al-Khansaa hospital during the period from the mid of February to the mid of April, 2005. Specimens were cultured on selected media and the growing colonies were identified using morphological characteristics and biochemical tests. Forty five bacterial isolates belongs to the family enterobacteriaceae were identified, 40%(18 isolates) of them were E.coli; 35.5% (16 isolates) were Klebsiella pneumoniae; 8.8%(4 isolates) of Proteus; 6.6% (3 isolates) of Citrobacter and 4.4% (two isolates) of Serratia and Salmonella. Protease production, which is considered as one of the factors enhancing inflammatory bowel disease, was investigated and it has been found that Serratia macescens , Proteus mirabilis , and Salmonella sp. were able to produce this enzyme while Klebsiella Pneumoniae didnt.