The Effect of Bioceramic-Based Root Canal Sealers on the Cell Viability and Cytotoxicity of Human Periodontal Ligament Fibroblast cell line (An in vitro study)

Section: Research Paper
Issue
Vol. 25 No. 1 (2025): Volume 25 Issue 1
Published
Mar 1, 2025
Pages
1-15

Abstract

Abstract: The current study aimed to assess the cell viability and cytotoxicity of bioceramic-based root canal sealers by comparing them to a zinc oxide-eugenol-based sealer (Endovit) in the set state of human periodontal ligament fibroblasts for one day, three days and seven days. Materials and Methods: Sealers were prepared following the manufacturer's directions and inserted in cylindrical rubber molds (5 mm diameter, 2 mm height) to get uniform sealer specimens, the specimens were kept for 24 hours at 37C. Human periodontal ligament fibroblasts were cultured with extracts from the tested sealers. Cell viability and Cytotoxicity were assessed for extracts of one-day, three-day days, and seven-day immersion. The cell viability of all root canal sealers was determined using an MTT test and cytotoxicity was determined using an LDH test. Data were statistically evaluated with One way-analysis of variance ANOVA followed by Duncans MRT to determine if group variance was significant. Results: Each tested sealer demonstrated a statistically significant variance in cell viability and cytotoxicity at various time intervals. NeoSEALER Flo had the highest cell viability and lowest cytotoxicity percentages followed by Cerafill RCS, MTA fillapex, and Endovit respectively at each evaluation time interval. Conclusions: NeoSEALER Flo was the least cytotoxic while ENDOVIT was the most cytotoxic root canal sealer. Cell viability decreased over time.

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Authors

Marwa Ahmed Jassim

Department of Conservative Dentistry, College of Dentistry, Mosul University, Mosul / Iraq

ORCID
Alaa E. Dawood

Melbourne Dental School, Oral Health CRC, The University of Melbourne, Melbourne, Vic. /Australia

ORCID
James Holden
ORCID
Eric Reynolds
ORCID

Identifiers

https://doi.org/10.33899/rdenj.2024.152429.1272
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