Isolation and Biochemical Study of the Active Proteinous Compounds from Coriandrum sativum Linn. Fruits

The research was an attempt to isolate and study the active protienous compounds from the cold aqueous extract of Coriandrum sativum L. using different biochemical techniques.Two compounds I and II were isolated using gel filtration chromatography of the precipitate produced by cold acetone precipitation.The comparative molecular weights of I and II were found to be in the range of 70800 and 4000 dalton respectively.
The work also included the study of the aqueous extract and the proteinous compounds on certain blood constituents and liver glycogen in normal and alloxan-induced diabetic mice. In normal mice, blood glucose level was lowered by concentrated aqueous extract and low molecular weight proteinous compound II to 54.4% and 50.4%. The aqueous extract and compound II also reduced the level of total cholesterol to 27.8% and 30.8%, whereas only the aqueous extract produced an increase in glycogen content in the liver of normal mice. In diabetic mice, the aqueous extract and compounds I and II lowered glucose level to 65.8%, 74.3% and 49.3% and also reduced the levels of total cholesterol and total lipids significantly. In addition, compound I showed a significant increase in glycogen content in liver of diabetic mice