Isolation and Identification of Plesiomonas Shigelloides and Study of Its Physiological Characteristics, Sensitivity of Antibiotics and Identifying Key

In an attempt to isolate Plesiomonas shigelliodes a total of 257 various specimens were collected. These includes 127 diarrhoeal stools, 50 sewage water, 50 tap water and 30 appendices. A total of 28 isolates of this bacteria were identified. The numbers of isolates and their percentages were 21 (16.5 %) in diarrhoeal specimens, 5 (5.1 %) from sewage water and 2 (6.7 %) from appendices. Other specimens were negative for this bacteria. The isolates were characterized by using standard identification tests and were found to be all cytochrome - oxidase positive, motile, inositol fermenters and glucose oxidizing fermenting and able of decarboxylation of amino acids. However, the isolates were negative to esculin, sheep blood haemolysis and production of Gelatinase, Lipase, DNase and Urease. They were - Lactamase positive. But sensitive to Gentamycin, Nalidixic acid and Ciprofloxacin. Through the project a most suitable transport and isolation media and flow sheet for the identification were suggested .