Indirect Spectrophotometric Method for Determination of Bromhexine-Hydrochloride in Pharmaceutical Preparations

A sensitive indirect spectrophotometric method is developed for the determination of bromhexine-HCl in pure and dosage forms. The method is based on the oxidation of bromhexine-HCl with iron(III) in acidic medium, then reaction of iron(II) with 1,10-phenathroline to produce a ferroin complex which is water-soluble, stable, and has a maximum absorption at 510 nm against the reagent blank with a molar absorptivity of 3.51104 l.mol-1.cm-1. Beers law is valid over the concentration range of 10 to 220g bromhexine-HCl in a final volume of 25 ml. The proposed method has been applied successfully to determine bromhexine-HCl in different pharmaceutical preparations.