Determination of the Best Transport and culture Media for First Isolation of Helicobacter pylori

The study sample consisted of 295 patients attending endoscopy unit of ibn-Sena teaching hospital in Mosul. These patients were complaining from epigastric pain, dyspepsia, acidity vomiting, flatulence and melena. Biopsies were taken from patients undergoing endoscopy and Transport, cultured Helicobacter pylori identification to evaluate the best transporting medium, listed as follows:(stuarts transport medium, normal saline (0.9%) and glucose solution (2%)) and evaluate the best transporting medium for the first isolation, listed as follows: (Brucella agar, Blood agar, Chocolate agar, Muller-Hintion agar, Eugon agar, Trypcase soy agar, Colombia agar base, Cystein trypcase agar, Wilkins Chalgren agar, Brain-Heart Infusion agar). It was found that the best media for biopsy transporting, the Stuarts transport medium. Its proportion in the term of isolation and growth was 85% with statistically significant difference (p<0.001). whereas Brucclla agar found to be the best medium for primary isolation with a statistically significant difference of 0.01 and 0.05 using F- test.