Extraction and diagnosis of fatty acids for Sinorhizobiummeliloti bacteria

The current study sought to investigate one of the determining factors of symbiosis between rhizobium bacteria and legumes (i.e. multi sugary acids of Sinorhizobium meliloti), where fatty acids on the lipid A chain were extracted and esterified for analysis using Gas-Liquid Chromatography (GLC) and determination. Moreover, a number of standard fatty acids were analyzed for comparison with fatty acids extracted from S. meliloti. Results show predominance of unsaturated Palmitoleic acid (C16:1) with percentage of 29.299. Furthermore, a number of saturated and unsaturated fatty acids, which levels exceeding model fatty acids analyzed within the scope of the study, were determined.