Extract and estimate the peroxidase enzyme from bean kales Phaseolus vulgaris L

Peroxidase enzyme was extracted from callus of different explantsand immature embryos of Phaseolus vulgaris L. .The maximum activityof enzyme showed in the embryos callus sample.The higher activity ofenzyme was in the ( 4months) age of callus for each of leaves ,roots andembryos and in (6months) for cotyledons callus,the stems callus recordedbest activity in (2months)old.Using of ammonium sulphate precipitationresult in high enzyme activity in all samples.When the exchangechromatochraphy was used to purifiy stems callus extract,(5)proteinapexes observed and ( 4 )of it was active .In the separation of proteins byusing the electrofousing techniques, the samples of embryos,leaves,stems,cotyledons and roots were separated at pH range (5.13-6.14 ).The minimum activity of enzyme observed at ( 4day) age for allexplants samples,but the maximum activity was in the roots samples at(14day)age