Thermal shock in increasing the content of protein and chlorophyll in Calus Carnation stalks Dianthus caryophyllus L

Exposure of callus derived from stem segments of carnation(Dianthus caryophyllus) to heat shock ( 30, 35 , 40, 45 , 50 o c) for 10and 20 minutes for each degree increased callus growth , fresh weight ,protein and chlorophyll content. Chlorophyll content reached up to 4.8mg /g in callus exposed to the treatment 30 c/20 min., other treatment30oc/10 min was found to stimulate protein content as compared with theuntreated callus.