The immunological effects of the polyamine oxidase enzyme partially cleared from the cerebrospinal fluid against secondary secondary cystitis in white mice. Coagulation factor and delayed hypersensitivity

The study included immune response to infection with secondaryhydatid disease in BALB/c mice activated by partially purifiedcerebrospinal fluid-polyamine oxidase (CSF-PAO) with spermine andinfected with protoscoleces of Echinococcus granulosus. Thepathological changes occurred in mice activated by differentconcentrations of PA0(200-1600 )lg/1 Ogm body weight) with constantconcentration (200)-lg/1 Ogm body weight) of substrate spermine, and bythe same concentration of spermine alone, were followed in comparisonwith positive control group along one month, depending on certaincriteria included changes in the non-specific and specific immuneresponse represented by phagocytosis and delayed-type hypersensitivity,respectively.The results revealed an increase in the non-specific (innate) andspecific (cellular) immune response, represented by an increase in the rateof phagocytic index and foot pad thickness, respectively, in activatedmice with PAO-Spm system, in comparison with +ve control group.Whereas an increase in the non-specific (innate), but no change inspecific (cellular) immune responses occurred with mice activated withSpm alone.It may , therefore, be concluded here that P AO isolated from CSFwith Spm could be considered as an effective immunumodulator againstinfection with secondary hydatid disease.