الطریقة الطیفیة لتحدید الکلورامفینیکول فی المستحضرات الصیدلانیة باستخدام کاشف الکلورانیل

ABSTRACT A simple, sensitive and rapid spectrophotometric method for the determination of chloramphenicol in pure as well as in dosage form is described. The method is based on the reaction of reduced chloramphenicol with o-chloranil in an aqueous alkaline medium to form a purple colored charge transfer complex of maximum absorption peak at 527 nm. Under the optimized reaction conditions, Beer’s law correlating the absorbance with chloramphenicol concentration was obeyed in the range of 2-65 μg ml-1 with molar absorptivity 3.72×103 L mol−1cm−1. The limits of detection and quantitation were 0.456 and 1.521 μg ml-1, respectively.   The accuracy and precision of the method were satisfactory; the average recovery was 100.9 % and values of relative standard deviations better than 2.2 %. The stoichiometry of the reaction was studied, and the reaction mechanism was postulated. The proposed method was successfully applied to the determination of chloramphenicol in its pharmaceutical capsule and eye drops with good accuracy and precisions. The results obtained by the proposed method are compared with those obtained by the official method and other reported methods.