Effect of crude alcohol extracts on some parts of clover plant Dianthus caryophllus in inhibition of fungal growth Fusarium culum and Fusarium oxysporum

Callus has been derived from Carnation leaves, on (MS) medium,Containing Various Concentrations of plant growth regulators, 0.1 mg/LBA and 0.5 mg/L 2,4-D. Established callus showed a good ability fordifferentiation and shoot formation on MS medium supplemented with3.0 mg/L BA and 0.1 mg/L IAA at a rate of 100%. Different rootingconcentrations were used with IBA in MS medium, 100% rooting wasobtained when IBA used at 1.0 mg/L. Phenols were separated from crudealcoholic extractions from leaves of seed plants, callus of leaves andleaves derived from regeneration plants. Phenols were identified byusing ferric chloride reagent. Treatment of both Fusarium oxysporumand Fusarium Culum with these extractions was successful in retardingthe growth of both fungi, by means of dry weight. The best crudealcoholic extract in retarding the growth of both fungi was found to be thecallus of leaves extracts which caused 67.7% and 62.1 retardation ingrowth of both fungi Fusarium oxysporum and Fusarium culumrespectively.