Effect of Garlic and Alcoholic Extract on the Mitrogenic Soralene Antifungal in Mushrooms Aspergillus amstelodami

This study includes testing the effect of four subinhibitoryconcentrations of garlic extracts (aqueous and alcoholic) on themutagenicity of 8-methoxypsoralen (8-MOP) in the presence of longwave ultraviolet (UV A) in conidia of the fungus Aspergillusamstelodami .The concentrations of aqueous extract were used in thisstudy are (2.5 , 5 , 7.5 and 10 mg I ml media ) and the concentrations ofalcoholic extracts are ( 0.5 , 1 , 1.5 and 2 mg I ml media) . Two protocolswere used to study the effect of garlic extracts on the mutagenicity of 8-MOP in the presence of UV A (366 nm) . In first protocol , thepretreatment method , all the concentrations (2.5 , 5 , 7.5 and 10 mg I ml )of aqueous extract increased the mutants frequencies which were inducedwith 8-MOP + UV A, but only the low concentration (0.5 mg I ml) of thealcoholic extract increases the frequency of the mutants induced with 8-MOP + UV A . In the second protocol , the growth mediated method ,both the aqueous and alcoholic extracts showed inhibitory effect on themutagenicity of 8-MOP + UV A.