The fungal effect of benomyl and phenolazoline in fungi and the production of cellulose enzyme and single cell protein Trichoder1na viride

This study was conducted aiming to obtain an efficient fungal isolatein cellulase and single cell protein production from a local isolateTrichoderma viride by the mutation effect of Benomyl and Vinclozolin.It was found that Benomyl has a lethal effect on the isolate at allconcentrations of the fungicide and spores suspension used, at the sametime it was found that Vinclozolin has an obvious mutational effect on theparental isolate at the concentration 2.0 mg active material/ml of standardmedium, the parental isolate showed clear morphological changes incolony shapes, colors, ability for growth in addition to the profoundchanges in fungal mycelia which was confirmed by the application ofslide culture technique.A qualitative and quantitative tests have been carried out to explorethe efTiciency of the new mutant for cellulase and single cell proteinlJroduction in solid and liquid media, it achieved cellulase specificactivity and single cell protein (3 .604 micromole/min/mgm protein and29.4 74% protein in dry weight) respectively.