Measure the ratios of fatty acids to some Gram negative bacteria

Fatty acids were analyzed from cell wall and cell membrane ofsome gram negative bacteria Pseudomonas aeruginosa, Plesimonasshigelloides and Proteus mirabilis by using gas liquid chromatography(GLC). Extraction and methylestrification analysis were done andcompared with standard fatty acids. The results showed that the majorpercent of fatty acids was lionleic acid (14.137o/o) in Ps. aeruginosafollowed by palmitic acid (10.059%) the other fatty acids percentmyrestic acid, stearic acid, oleic acid, capric acid, lauric acid weredifferent and it was 0.527%, 8.042%, 6.261%, 1.205%, 0.527%respectively. While the higher percent of fatty acids in Plesimonasshigelloides was palmitic acid (20.621 %) followed by oleic acid(15.816%), stearic acid (15.373%) and myrestic acid (10.897%). Theminor percent which was less than 1% belonged to lauric acid, capricacid, docosahexanoic acid, eicosapentanoic. For Proteus mirabilis,myristic acid and lauric acid appeared with higher percent 17.349%and13.130% respectively.