The use of cyclohexymide and chloramphenicol in raising the resistance of wheat plants triticum estivunz L. to cold and ablution
Abstract
Homogenized healthy seeds of Triticum aestivum L .Abo -Greab-3-cultivar were used to study the possibility of using a mixture oftwo protein synthesis inhibitors in Eukaryote and Prokaryote (cycloheximide and hloramphenicol )to increase cold and freezingtolerance of wheat . The study was designed as (3x3x3) Factorialexperiment and carried -out with three replicates according to randomizecomplete block design (RCBD). The first factor was represented by thetemperature which comprised 20,o,-5 C0 The second factor was theconcentration of the antibiotics mixture (cycloheximidechloramphenicol) which comprised 0:0,50}1g/mL:50 }lg/mL and 100}lg/mL: 100 }lglmL. The third factor was the treatment periods which were0,12,and 24 hours .Different degrees of temperature showed significant effects at0.05 level of significance in: plant height, nu .of tillers I plant ,nu. ofspikes /plant ,length and weight of spikes and weight of 1000 grains.Different concenterations of cycloheximide and chloramphenicol mixtureshowed significant effects in :nu .of tillers /plant ,nu. of spikes/ plant,weight of spikes ,nu. of grains /spikes and 1000 grains weight .Treatmentperiods showed significant effects in all studied parameters except infresh and dry gluten.All the interactions among the studied factors were significant.