Isolation of alesin as a product of secondary metabolism of kales plant Allium sativum

This study successed in isolating the secondary metabolicproduct named allicin .This compound was isolated from various callus cultures & garlicplants produced from seeds as well .The isolated material was identified using thin layer chromatography(T.L.C.) , UV Spectrophotometer & infra red techniques .The results revealed that a single compound was isolated from allalcoholic plant extracts . the unique compound isolated from all sampleshas the same flow - rate and possess identical chemical structure whenmatched with standard allicin.The finding that callus culture contains high level of allicin up to200 mg I 1 Og fresh weight , while garlic cloves contain 20 mg I 1 Ogfresh weight which is known to be rich with allicin.This study described the effect of different photoperiod includingcontinuous light or continuous dark and culture medium type on theaccumulation of allicin .The results confirmed that callus tissue grown onMS medium enhanced the allicin quantity that reached up to 200 mg I1 Og fresh weight of callus incubated in continuous light for two weeks .